An aptamer-guided fluorescence polarisation platform for extracellular vesicle liquid biopsy.

ABSTRACT: The translation of discoveries on extracellular vesicle (EV) based cancer biomarkers to personalised precision oncology requires the development of robust, sensitive and specific assays that are amenable to adoption in the clinical laboratory. Whilst a variety of elegant approaches for EV liquid biopsy have been developed, most of them remain as research prototypes due to the requirement of a high level of microfabrication and/or sophisticated instruments. Hence, this study is set to develop a simple DNA aptamer-enabled and fluorescence polarisation-based homogenous assay that eliminates the need to separate unbound detection ligands from the bound species for EV detection. High specificity is achieved by immobilising EVs with one set of antibodies and subsequently detecting them with a DN

SUBMITTER: Pham CV 

PROVIDER: S-EPMC11367152 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature