Genome binding properties of Zic transcription factors underlie their changing functions during neuronal maturation.

ABSTRACT:

Background

The Zic family of transcription factors (TFs) promote both proliferation and maturation of cerebellar granule neurons (CGNs), raising the question of how a single, constitutively expressed TF family can support distinct developmental processes. Here we use an integrative experimental and bioinformatic approach to discover the regulatory relationship between Zic TF binding and changing programs of gene transcription during postnatal CGN differentiation.

Results

We first established a bioinformatic pipeline to integrate Zic ChIP-seq data from the developing mouse cerebellum with other genomic datasets from the same tissue. In newborn CGNs, Zic TF binding predominates at active enhancers that are co-bound by developmentally regulated TFs including Atoh1, whereas in m

SUBMITTER: Minto MS 

PROVIDER: S-EPMC11367862 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature