Prostatic lineage differentiation from human embryonic stem cells through inducible expression of NKX3-1.

ABSTRACT:

Background

Understanding the lineage differentiation of human prostate not only is crucial for basic research on human developmental biology but also significantly contributes to the management of prostate-related disorders. Current knowledge mainly relies on studies on rodent models, lacking human-derived alternatives despite clinical samples may provide a snapshot at certain stage. Human embryonic stem cells can generate all the embryonic lineages including the prostate, and indeed a few studies demonstrate such possibility based on co-culture or co-transplantation with urogenital mesenchyme into mouse renal capsule.

Methods

To establish a stepwise protocol to obtain prostatic organoids in vitro from human embryonic stem cells, we apply chemicals and growth factors by mimi

SUBMITTER: Wang S 

PROVIDER: S-EPMC11367998 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature