Strangers in a foreign land: 'Yeastizing' plant enzymes.

ABSTRACT: Expressing plant metabolic pathways in microbial platforms is an efficient, cost-effective solution for producing many desired plant compounds. As eukaryotic organisms, yeasts are often the preferred platform. However, expression of plant enzymes in a yeast frequently leads to failure because the enzymes are poorly adapted to the foreign yeast cellular environment. Here, we first summarize the current engineering approaches for optimizing performance of plant enzymes in yeast. A critical limitation of these approaches is that they are labour-intensive and must be customized for each individual enzyme, which significantly hinders the establishment of plant pathways in cellular factories. In response to this challenge, we propose the development of a cost-effective computational pipeline to

SUBMITTER: Van Gelder K 

PROVIDER: S-EPMC11368087 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature