Penicillin-binding protein 3 sequence variations reduce susceptibility of Pseudomonas aeruginosa to β-lactams but inhibit cell division.

ABSTRACT:

Background

β-lactam antibiotics, which inhibit penicillin-binding protein 3 (PBP3) that is required for cell division, play a key role in treating P. aeruginosa infections. Some sequence variations in PBP3 have been associated with β-lactam resistance but the effects of variations on antibiotic susceptibility and on cell division have not been quantified. Antibiotic efflux can also reduce susceptibility.

Objectives

To quantify the effects of PBP3 variations on β-lactam susceptibility and cell morphology in P. aeruginosa.

Methods

Nineteen PBP3 variants were expressed from a plasmid in the reference strain P. aeruginosa PAO1 and genome engineering was used to construct five mutants expressing PBP3 variants from the chromosome. The effects of the variations on β-lactam m

SUBMITTER: Glen KA 

PROVIDER: S-EPMC11368433 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature