Cell size regulates human endoderm specification through actomyosin-dependent AMOT-YAP signaling.

ABSTRACT: Cell size is a crucial physical property that significantly impacts cellular physiology and function. However, the influence of cell size on stem cell specification remains largely unknown. Here, we investigated the dynamic changes in cell size during the differentiation of human pluripotent stem cells into definitive endoderm (DE). Interestingly, cell size exhibited a gradual decrease as DE differentiation progressed with higher stiffness. Furthermore, the application of hypertonic pressure or chemical to accelerate the reduction in cell size significantly and specifically enhanced DE differentiation. By functionally intervening in mechanosensitive elements, we have identified actomyosin activity as a crucial mediator of both DE differentiation and cell size reduction. Mechanistically, th

SUBMITTER: Jiang L 

PROVIDER: S-EPMC11368700 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature