Proteomic analysis of serum small extracellular vesicles identifies diagnostic biomarkers for neuroblastoma.

ABSTRACT:

Background

Neuroblastoma (NB) primarily arises in children who are <10 years of age, and originates from developing sympathetic nervous system, which results in tumors in adrenal glands and/or sympathetic ganglia. The diagnosis of NB involves a combination of laboratory and imaging tests, and biopsies. Small extracellular vesicles (sEVs) have gained attention as potential biomarkers for various types of tumors. Here, we performed proteomic analysis of serum sEVs and identified potential biomarkers for NB.

Methods

Label-free proteomics of serum sEVs were performed in the discovery phase. A bulk RNA-seq dataset of NB tissues was used to analyze the association between genes encoding sEVs proteins and prognosis. Potential biomarkers were validated via multiple reaction monitori

SUBMITTER: Cheng J 

PROVIDER: S-EPMC11368728 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature