PET imaging of Aspergillus infection using Zirconium-89 labeled anti-β-glucan antibody fragments.

ABSTRACT:

Purpose

Invasive fungal diseases, such as pulmonary aspergillosis, are common life-threatening infections in immunocompromised patients and effective treatment is often hampered by delays in timely and specific diagnosis. Fungal-specific molecular imaging ligands can provide non-invasive readouts of deep-seated fungal pathologies. In this study, the utility of antibodies and antibody fragments (Fab) targeting β-glucans in the fungal cell wall to detect Aspergillus infections was evaluated both in vitro and in preclinical mouse models.

Methods

The binding characteristics of two commercially available β-glucan antibody clones and their respective antigen-binding Fabs were tested using biolayer interferometry (BLI) assays and immunofluorescence staining. In vivo binding of the

SUBMITTER: Lai J 

PROVIDER: S-EPMC11368974 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature