Concentration of voxelotor in sickle cell disease can be estimated using electrophoresis and high-performance liquid chromatography.

ABSTRACT:

Objectives

Voxelotor can increase hemoglobin levels in patients living with sickle cell disease (SCD). A clinician who is monitoring voxelotor response may want to know whole-blood voxelotor concentration, but this cannot be measured in most clinical settings. However, voxelotor has been demonstrated to cause "peak splitting" in common methods of hemoglobin measurement such as capillary zone electrophoresis (CZE) and high-performance liquid chromatography (HPLC). We hypothesized that we could use the size of the peak split to estimate the whole-blood concentration.

Methods

Blood from people with SCD was dosed with known concentrations of voxelotor, and multiparameter regression was used to derive the relationship of voxelotor concentration to the degree of peak splitting obs

SUBMITTER: Curtis SA 

PROVIDER: S-EPMC11369063 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature