Identification of the oosporein biosynthesis gene cluster in an entomopathogenic fungus Blackwellomyces cardinalis.

ABSTRACT: Blackwellomyces cardinalis (≡ Cordyceps cardinalis) is an entomopathogenic fungus that hosts lepidopteran insect larvae. Oosporein, produced by Bl. cardinalis, is a red secondary metabolite that is also produced by other entomopathogens and is known to contribute to entomopathogenic activity. In this study, a homologous region of the oosporein biosynthesis gene cluster (BcOpS cluster) was found from the genome sequence of Bl. cardinalis strain NBRC 103832. Within the cluster, a putative transcription factor gene BcOpS3 was deleted by homologous recombination. The deletion strain (ΔBcOpS3) did not produce oosporein. Real-time qPCR analysis showed that the expression of all genes was either lost or greatly reduced compared to the wild type str

SUBMITTER: Nakamura Y 

PROVIDER: S-EPMC11369306 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature