Pollen-expressed RLCKs control pollen tube burst.

ABSTRACT: In angiosperms, the pollen tube enters the receptive synergid cell, where it ruptures to release its cytoplasm along with two sperm cells. This interaction is complex, and the exact signal transducers that trigger the bursting of pollen tubes are not well understood. In this study, we identify three homologous receptor-like cytoplasmic kinases (RLCKs) expressed in pollen tubes of Arabidopsis, Delayed Burst 1/2/3 (DEB1/2/3), which play a crucial role in this process. These genes produce proteins localized on the plasma membrane, and their knockout causes delayed pollen tube burst and entrance of additional pollen tubes into the embryo sac due to fertilization recovery. We show that DEBs interact with the Ca²⁺ pump ACA9, influencing the dynamics of cytoplasmic Ca²⁺ in p

SUBMITTER: Xu YJ 

PROVIDER: S-EPMC11369774 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature