Optimizing Promoters and Subcellular Localization for Constitutive Transgene Expression in Marchantia polymorpha.

ABSTRACT: Marchantia polymorpha has become an important model system for comparative studies and synthetic biology. The systematic characterization of genetic elements would make heterologous gene expression more predictable in this test bed for gene circuit assembly and bioproduction. Yet, the toolbox of genetic parts for Marchantia includes only a few constitutive promoters that need benchmarking to assess their utility. We compared the expression patterns of previously characterized and new constitutive promoters. We found that driving expression with the double enhancer version of the cauliflower mosaic virus 35S promoter (pro35S × 2) provided the highest yield of proteins, although it also inhibits the growth of transformants. In contrast, promoters derived from the Marchantia genes for ETHYLEN

SUBMITTER: Tse SW 

PROVIDER: S-EPMC11369823 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature