Emergence of antibiotic-specific <i>Mycobacterium tuberculosis</i> phenotypes during prolonged treatment of mice.

ABSTRACT: A major challenge in tuberculosis (TB) therapeutics is that antibiotic exposure leads to changes in the physiologic state of M. tuberculosis (Mtb) which may enable the pathogen to withstand treatment. While antibiotic-treated Mtb have been evaluated in short-term in vitro experiments, it is unclear if and how long-term in vivo treatment with diverse antibiotics with varying treatment-shortening activity (sterilizing activity) affect Mtb physiologic states differently. Here, we used SEARCH-TB, a pathogen-targeted RNA-sequencing platform, to characterize the Mtb transcriptome in the BALB/c high-dose aerosol infection mouse model following 4-week treatment with three sterilizing and three non-sterilizing antibiotics. Certain transcriptional cha

SUBMITTER: Wynn EA 

PROVIDER: S-EPMC11370397 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature