High-throughput affinity measurements of direct interactions between activation domains and co-activators.

ABSTRACT: Sequence-specific activation by transcription factors is essential for gene regulation^1,2. Key to this are activation domains, which often fall within disordered regions of transcription factors^3,4 and recruit co-activators to initiate transcription⁵. These interactions are difficult to characterize via most experimental techniques because they are typically weak and transient^6,7. Consequently, we know very little about whether these interactions are promiscuous or specific, the mechanisms of binding, and how these interactions tune the strength of gene activation. To address these questions, we developed a microfluidic platform for expression and purification of hundreds of activation domains in parallel followed by direct measurement of co-acti

SUBMITTER: DelRosso N 

PROVIDER: S-EPMC11370418 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature