Description of Bacterial RNA Transcripts Detected in <i>Mycobacterium tuberculosis</i> - Infected Cells from Peripheral Human Granulomas using Single Cell RNA Sequencing.

ABSTRACT: Mycobacterium tuberculosis (Mtb) remains a global human health threat and a significant cause of human morbidity and mortality. We document here the capture of Mtb transcripts in libraries designed to amplify eukaryotic mRNA. These reads are often considered spurious or nuisance and are rarely investigated. Because of early literature suggesting the possible presence of polyadenylated transcripts in Mtb RNA, we included the H37Rv Mtb reference genome when assembling scRNA seq libraries from fine needle aspirate samples from patients presenting at the TB clinic, Port Moresby General Hospital, Papua New Guinea. We used 10X Genomics single-cell RNA sequencing transcriptomics pipeline, which initiates mRNA amplification with poly-T primers on ~30-micron beads designed to capture, in thi

SUBMITTER: Moos PJ 

PROVIDER: S-EPMC11370423 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature