Whole-brain mapping in adult zebrafish and identification of a novel tank test functional connectome.

ABSTRACT: Identifying general principles of brain function requires the study of structure-function relationships in a variety of species. Zebrafish have recently gained prominence as a model organism in neuroscience, yielding important insights into vertebrate brain function. Although methods have been developed for mapping neural activity in larval animals, we lack similar techniques for adult zebrafish that have the advantage of a fully developed neuroanatomy and larger behavioral repertoire. Here, we describe a pipeline built around open-source tools for whole-brain activity mapping in freely swimming adult zebrafish. Our pipeline combines recent advances in histology, microscopy, and machine learning to capture cfos activity across the entirety of the adult brain. Images captured using l

SUBMITTER: Rajput N 

PROVIDER: S-EPMC11370427 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature