Detection, Isolation and Quantification of Myocardial Infarct with Four Different Histological Staining Techniques.

ABSTRACT: Precise quantification of myocardial infarction is crucial for evaluating the therapeutic strategies. We developed a robust, color-based semi-automatic algorithm capable of infarct region detection, isolation and quantification with four different histological staining techniques, and the isolation and quantification of diffuse fibrosis in the heart. Our method is developed based on the color difference in the infarct and non-infarct regions after histological staining. Mouse cardiac tissues stained with Masson's trichrome (MTS), hematoxylin and eosin (H&E), 2,3,5-Triphenyltetrazolium chloride and picrosirius red were included to demonstrate the performance of our method. We demonstrate that our algorithm can effectively identify and produce a clear visualization of infarct tissue for the

SUBMITTER: Wu X 

PROVIDER: S-EPMC11370443 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature