Sensitive and modular amplicon sequencing of <i>Plasmodium falciparum</i> diversity and resistance for research and public health.

ABSTRACT:

Background

Targeted amplicon sequencing is a powerful and efficient tool for interrogating the Plasmodium falciparum genome, generating actionable data from infections to complement traditional malaria epidemiology. For maximum impact, genomic tools should be multi-purpose, robust, sensitive, and reproducible.

Methods

We developed, characterized, and implemented MAD⁴HatTeR, an amplicon sequencing panel based on Multiplex Amplicons for Drug, Diagnostic, Diversity, and Differentiation Haplotypes using Targeted Resequencing, along with a bioinformatic pipeline for data analysis. Additionally, we introduce an analytical approach to detect gene duplications and deletions from amplicon sequencing data. Laboratory control and field samples were used to demonstrate

SUBMITTER: Aranda-Diaz A 

PROVIDER: S-EPMC11370457 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature