Lipid- and protein-directed photosensitizer proximity labeling captures the cholesterol interactome.

ABSTRACT: The physical properties of cellular membranes, including fluidity and function, are influenced by protein and lipid interactions. In situ labeling chemistries, most notably proximity-labeling interactomics are well suited to characterize these dynamic and often fleeting interactions. Established methods require distinct chemistries for proteins and lipids, which limits the scope of such studies. Here we establish a singlet-oxygen-based photocatalytic proximity labeling platform (POCA) that reports intracellular interactomes for both proteins and lipids with tight spatiotemporal resolution using cell-penetrant photosensitizer reagents. Using both physiologically relevant lipoprotein-complexed probe delivery and genetic manipulation of cellular cholesterol handling machinery, cholesterol-dir

SUBMITTER: Becker AP 

PROVIDER: S-EPMC11370482 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature