Proton pump inhibitors modulate esophageal epithelial barrier function and crosstalk with eosinophils.

ABSTRACT:

Background

Eosinophilic esophagitis (EoE) is a chronic allergic disease characterized by esophageal dysfunction, type-2 inflammation, and esophageal eosinophilic infiltrate. While proton pump inhibitor (PPI) therapy is commonly used for EoE management, the underlying mechanism of action remains unclear.

Methods

Air-liquid interface culture of esophageal epithelial cells was employed to investigate the impact of the PPI omeprazole on barrier integrity in IL-13-treated cultures. Epithelial chemokine secretion was assessed following stimulation with IL-13 and omeprazole, and the migration of eosinophils from healthy human donors was evaluated using 3 μm pore-sized transwells. A co-culture system of epithelial cells and eosinophils was employed to study chemokine secretion and e

SUBMITTER: Gautam R 

PROVIDER: S-EPMC11370561 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature