Microenvironment T-Type calcium channels regulate neuronal and glial processes to promote glioblastoma growth.

ABSTRACT:

Background

Glioblastoma (GBM) is the most common primary malignant brain tumor. The aim of this study was to elucidate the role of microenvironment and intrinsic T-type calcium channels (Cav3) in regulating tumor growth and progression.

Methods

We grafted syngeneic GBM cells into Cav3.2 knockout mice to assess the role of microenvironment T-Type calcium channels on GBM tumor growth. We performed single-cell RNA-seq (scRNA-seq) of tumors from WT and Cav3.2 KO mice to elucidate the regulation of tumors by the microenvironment. We used neurons from WT and Cav3.2 KO mice in co-culture with GBM stem cells (GSC) to assess the effects of Cav3.2 on neuron/GSC synaptic connections and tumor cell growth.

Results

Cav3.2 KO in the microenvironment led to significant reduction of

SUBMITTER: Dube CJ 

PROVIDER: S-EPMC11370607 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature