DNA methylation of miR-181a-5p mediated by DNMT3b drives renal interstitial fibrosis developed from acute kidney injury.
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ABSTRACT:
SUBMITTER: Liu H
PROVIDER: S-EPMC11370974 | biostudies-literature | 2024
REPOSITORIES: biostudies-literature
Publications
DNA methylation of miR-181a-5p mediated by DNMT3b drives renal interstitial fibrosis developed from acute kidney injury.
Liu Huaxi H Deng Yijian Y Luo Guanfeng G Yang Ying Y Xie Bei B Diao Huiling H Chen Meilin M Chen Liqian L Xie Penghui P Kwan Hiu Yee HY Zhao Xiaoshan X Sun Xiaomin X
Epigenomics 20240718 13
<b>Aim:</b> To explore the role of miR-181a-5p in the progression of acute kidney injury (AKI) to renal interstitial fibrosis (RIF) from the perspective of DNA methylation.<b>Materials & methods:</b> The role of miR-181a-5p was confirmed by collecting clinical samples, injecting miR-181a-5p agomir into tail vein, and transfecting miR-181a-5p mimic <i>in vitro</i>. The mechanism of miR-181a-5p's influence on AKI induced RIF was investigated by methylation-specific PCR, bioinformatic analysis, tra ...[more]
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