Human Conjunctival Transcriptome in Acanthamoeba Keratitis: An Exploratory Study.

ABSTRACT:

Purpose

The purpose of this study was to identify conjunctival transcriptome differences in patients with Acanthamoeba keratitis compared with keratitis with no known associated pathogen.

Methods

The host conjunctival transcriptome of 9 patients with Acanthamoeba keratitis (AK) is compared with the host conjunctival transcriptome of 13 patients with pathogen-free keratitis. Culture and/or confocal confirmed Acanthamoeba in 8 of 9 participants with AK who underwent metagenomic RNA sequencing as the likely pathogen. Cultures were negative in all 13 cases where metagenomic RNA sequencing did not identify a pathogen.

Results

Transcriptome analysis identified 36 genes differently expressed between patients with AK and patients with presumed sterile, or pathogen-free, kerat

SUBMITTER: Seitzman GD 

PROVIDER: S-EPMC11371541 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature