Rapid and sensitive detection of methicillin-resistant Staphylococcus aureus through the RPA-PfAgo system.

ABSTRACT:

Introduction

Both the incidence and mortality rates associated with methicillin-resistant Staphylococcus aureus (MRSA) have progressively increased worldwide. A nucleic acid testing system was developed in response, enabling swift and precise detection of Staphylococcus aureus (S. aureus) and its MRSA infection status. This facilitates improved prevention and control of MRSA infections.

Methods

In this work, we introduce a novel assay platform developed by integrating Pyrococcus furiosus Argonaute (PfAgo) with recombinase polymerase amplification (RPA), which was designed for the simultaneous detection of the nuc and mecA genes in MRSA.

Results

This innovative approach enables visual MRSA detection within 55 mins, boast

SUBMITTER: Chen W 

PROVIDER: S-EPMC11371615 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature