Rapid alignment-free bacteria identification via optical scattering with LEDs and YOLOv8.

ABSTRACT: Rapid and accurate bacterial identification is essential for timely treatment of infections like sepsis. While traditional methods are reliable, they lack speed, and advanced molecular techniques often suffer from cost and complexity. The bacterial detection technology based on optical scattering system offers a rapid, label-free alternative but traditionally relies on complex lasers and analysis. Our enhanced approach utilizes RGB light emitting diodes (LEDs) as the light source. Three diffraction images of bacterial colonies from different LED colors are separately captured by a USB camera and combined using an image registration algorithm to enhance image sharpness. Our approach utilizes an object detection model, i.e., YOLOv8, for analysis achieving high-accuracy differentiation betwee

SUBMITTER: Romphosri S 

PROVIDER: S-EPMC11371926 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature