The natural compound periplogenin suppresses the growth of prostate carcinoma cells by directly targeting ATP1A1.

ABSTRACT: Natural compounds constitute a major resource for the development of medicines for multiple diseases. While many natural compounds show strong biological activity, the mechanisms that confer clinical benefits are often elusive and have been attributed to multiple pathways. Periplogenin (PPG), a natural compound isolated from Cortex periplocae, exhibits strong anti-tumor activities in several human cancer cell lines. However, its molecular mode of action remained unclear. In this study, we leveraged a forward genetic screening approach in DU145 prostate cancer cells to uncover the molecular target of PPG using chemical mutagenesis. Next generation sequencing revealed that a single amino acid substitution at amino acid 804 in ATP1A1 (ATPase Na + /K + Transporting Subunit Alpha 1) confers res

SUBMITTER: Zhang X 

PROVIDER: S-EPMC11372130 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature