A neutrophil elastase-generated mature form of IL-33 is a potent regulator of endothelial cell activation and proliferative retinopathy.

ABSTRACT: Human interleukin-33 (IL-33) is a 270 amino acid protein that belongs to the IL-1 cytokine family and plays an important role in various inflammatory disorders. Neutrophil proteases (Cathepsin G and Elastase) and mast cell proteases (tryptase and chymase) regulate the activity of IL-33 by processing full-length IL-33 into its mature form. There is little evidence on the role of these mature forms of IL-33 in retinal endothelial cell signaling and pathological retinal angiogenesis. Here, we cloned, expressed, and purified the various mature forms of human IL-33 and then evaluated the effects of IL-33_95-270, IL-33_99-270, IL-33_109-270, and IL-33_112-270 on angiogenesis in human retinal microvascular endothelial cells (HRMVECs). We observed that IL-33

SUBMITTER: Bisen S 

PROVIDER: S-EPMC11372157 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature