Impact of synthesis methods on the functionality of antibody-conjugated gold nanoparticles for targeted therapy.

ABSTRACT: Gold nanoparticles (GNPs) are emerging as promising modular platforms for antibody-based cancer therapeutics. Their unique physiochemical properties enable efficient binding of multiple antibodies upon a single particle, thereby enhancing therapeutic potential. However, the effect of widely used synthesis techniques on the characteristics and functionality of antibody-GNP platforms has yet to be fully understood. Here, we investigated the effect of key synthesis approaches, namely, covalent binding and physical adsorption, on the properties and anti-cancer functionality of antibody-coated GNPs. By carefully manipulating synthesis variables, including antibody mass in reaction and linker compositions, we revealed a direct impact of these synthesis methods on antibody binding efficiency and

SUBMITTER: Anaki A 

PROVIDER: S-EPMC11372556 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature