Greener and whiter analytical method development and validation for determining the presence of zolpidem tartrate infused in apple juice using RP-HPLC via magnetic solid-phase extraction followed by LC-MS confirmatory analysis.

ABSTRACT: The research work entails a newly developed RP-HPLC method, aimed at analyzing the modern date rape drug, zolpidem tartrate (ZT), infused in apple juice matrix. The work relies on dispersive solid-phase extraction (DSPE) with polyethylene imine (PEI)-coated magnetic nanoparticles to preconcentrate zolpidem from the matrix, in the presence of trifluoroacetic acid (TFA) for matrix isolation, for the first time. The optimized conditions emphasize the use of an environmentally preferable mobile phase [methanol: 0.5% acetic acid (60 : 40% v/v; pH 2.50)] at a 1 ml min^-1 flow rate, employed with a Platisil Octa-Decyl Silane (ODS) column (250 × 4.6 mm; 5 μm). Further, the validated results were confirmed to be within the ICH guidelines, marking the method demonstrated to be linear (R

SUBMITTER: Sundara Moorthy R 

PROVIDER: S-EPMC11372563 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature