The Immobilization of a Cyclodipeptide Synthase Enables Biocatalysis for Cyclodipeptide Production.

ABSTRACT: Cyclodipeptide synthases (CDPSs) are enzymes that use aminoacylated tRNAs as substrates to produce cyclic dipeptide natural products acting as anticancer and neuroprotective compounds. Many CDPSs, however, suffer from instability and poor recyclability, while enzyme immobilization can enhance catalyst efficiency and reuse. Here, the CDPS enzyme from Parcubacteria bacterium RAAC4_OD1_1 was immobilized using three different supports: biochar from waste materials, calcium-alginate beads, and chitosan beads. Immobilization of active PbCDPS was successful, and production of the cyclodipeptide cyclo (His-Glu) (cHE) was confirmed by HPLC-MS. Biochar from spent coffee activated with glutaraldehyde, alginate beads, and chitosan beads activated with glutaraldehyde led to a 5-fold impro

SUBMITTER: Alvarado-Ramirez L 

PROVIDER: S-EPMC11372833 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature