Single cell RNA-sequencing delineates CD8⁺ tissue resident memory T cells maintaining rejection in liver transplantation.

ABSTRACT: Rationale: Understanding the immune mechanisms associated with liver transplantation (LT), particularly the involvement of tissue-resident memory T cells (TRMs), represents a significant challenge. Methods: This study employs a multi-omics approach to analyse liver transplant samples from both human (n = 17) and mouse (n = 16), utilizing single-cell RNA sequencing, bulk RNA sequencing, and immunological techniques. Results: Our findings reveal a comprehensive T cell-centric landscape in LT across human and mouse species, involving 235,116 cells. Notably, we found a substantial increase in CD8⁺ TRMs within rejected grafts compared to stable ones. The elevated presence of CD8⁺ TRMs is characterised by a distinct expression profile, featuring upregul

SUBMITTER: Li X 

PROVIDER: S-EPMC11373625 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature