In-depth cross-validation of human and mouse CD4-specific minibodies for noninvasive PET imaging of CD4⁺ cells and response prediction to cancer immunotherapy.

ABSTRACT: Increasing evidence emphasizes the pivotal role of CD4⁺ T cells in orchestrating cancer immunity. Noninvasive in vivo imaging of the temporal dynamics of CD4⁺ T cells and their distribution patterns might provide novel insights into their effector and regulator cell functions during cancer immunotherapy (CIT). Methods: We conducted a comparative analysis of ⁸⁹Zr-labeled anti-mouse (m) and anti-human (h) CD4-targeting minibodies (Mbs) for in vivo positron emission tomography (PET)/magnetic resonance imaging (MRI) of CD4⁺ T cells in human xenografts, syngeneic tumor-bearing wild-type (WT), and human CD4⁺ knock-in (hCD4-KI) mouse models. Results: Both ⁸⁹Zr-CD4-Mbs yielded high radiolabeling efficienc

SUBMITTER: Pezzana S 

PROVIDER: S-EPMC11373626 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature