Impact of tRNA-induced proline-to-serine mistranslation on the transcriptome of Drosophila melanogaster.

ABSTRACT: Mistranslation is the misincorporation of an amino acid into a polypeptide. Mistranslation has diverse effects on multicellular eukaryotes and is implicated in several human diseases. In Drosophila melanogaster, a serine transfer RNA (tRNA) that misincorporates serine at proline codons (P→S) affects male and female flies differently. The mechanisms behind this discrepancy are currently unknown. Here, we compare the transcriptional response of male and female flies to P→S mistranslation to identify genes and cellular processes that underlie sex-specific differences. Both males and females downregulate genes associated with various metabolic processes in response to P→S mistranslation. Males downregulate genes associated with extracellular matrix organization and response to negative stimuli

SUBMITTER: Isaacson JR 

PROVIDER: S-EPMC11373654 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature