Polyethylene glycol and caspase inhibitor emricasan alleviate cold injury in primary rat hepatocytes.

ABSTRACT: Current methods of storing explanted donor livers at 4 °C in University of Wisconsin (UW) solution result in loss of graft function and ultimately lead to less-than-ideal outcomes post transplantation. Our lab has previously shown that supplementing UW solution with 35-kilodalton polyethylene glycol (PEG) has membrane stabilizing effects for cold stored primary rat hepatocytes in suspension. Expanding on past studies, we here investigate if PEG has the same beneficial effects in an adherent primary rat hepatocyte cold storage model. In addition, we investigated the extent of cold-induced apoptosis through treating cold-stored hepatocytes with pan caspase inhibitor emricasan. In parallel to storage at the current cold storage standard of 4 °C, we investigated the effects of lowering the sto

SUBMITTER: Chen H 

PROVIDER: S-EPMC11374468 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature