A novel micropeptide, Slitharin, exerts cardioprotective effects in myocardial infarction.

ABSTRACT:

Purpose

Micropeptides are an emerging class of proteins that play critical roles in cell signaling. Here, we describe the discovery of a novel micropeptide, dubbed slitharin (Slt), in conditioned media from Cardiosphere-derived cells (CDCs), a therapeutic cardiac stromal cell type.

Experimental design

We performed mass spectrometry of peptide-enriched fractions from the conditioned media of CDCs and a therapeutically inert cell type (human dermal fibrobasts). We then evaluated the therapeutic capacity of the candidate peptide using an in vitro model of cardiomyocyte injury and a rat model of myocardial infarction.

Results

We identified a novel 24-amino acid micropeptide (dubbed Slitharin [Slt]) with a non-canonical leucine start codon, arising from long intergenic non

SUBMITTER: Ibrahim AGE 

PROVIDER: S-EPMC11374934 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature