Nanodynamo quantifies subcellular RNA dynamics revealing extensive coupling between steps of the RNA life cycle.

ABSTRACT: The coordinated action of transcriptional and post-transcriptional machineries shapes gene expression programs at steady state and determines their concerted response to perturbations. We have developed Nanodynamo, an experimental and computational workflow for quantifying the kinetic rates of nuclear and cytoplasmic steps of the RNA life cycle. Nanodynamo is based on mathematical modelling following sequencing of native RNA from cellular fractions and polysomes. We have applied this workflow to triple-negative breast cancer cells, revealing widespread post-transcriptional RNA processing that is mutually exclusive with its co-transcriptional counterpart. We used Nanodynamo to unravel the coupling between transcription, processing, export, decay and translation machineries. We have identifi

SUBMITTER: Coscujuela Tarrero L 

PROVIDER: S-EPMC11375098 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature