Extracting regulatory active chromatin footprint from cell-free DNA.

ABSTRACT: Cell-free DNA (cfDNA) has emerged as a pivotal player in precision medicine, revolutionizing the diagnostic and therapeutic landscape. While its clinical applications have significantly increased in recent years, current cfDNA assays have limited ability to identify the active transcriptional programs that govern complex disease phenotypes and capture the heterogeneity of the disease. To address these limitations, we have developed a non-invasive platform to enrich and examine the active chromatin fragments (cfDNA_ac) in peripheral blood. The deconvolution of the cfDNA_ac signal from traditional nucleosomal chromatin fragments (cfDNA_nuc) yields a catalog of features linking these circulating chromatin signals in blood to specific regulatory elements across th

SUBMITTER: Lai K 

PROVIDER: S-EPMC11375110 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature