The compartment-specific manipulation of the NAD⁺/NADH ratio affects the metabolome and the function of glioblastoma.

ABSTRACT: Glioblastoma multiforme (GBM) is the most aggressive type of cancer in the brain and has an inferior prognosis because of the lack of suitable medicine, largely due to its tremendous invasion. GBM has selfish metabolic pathways to promote migration, invasion, and proliferation compared to normal cells. Among various metabolic pathways, NAD (nicotinamide adenine dinucleotide) is essential in generating ATP and is used as a resource for cancer cells. LbNOX (Lactobacillus brevis NADH oxidase) is an enzyme that can directly manipulate the NAD⁺/NADH ratio. In this study, we found that an increased NAD⁺/NADH ratio by LbNOX or mitoLbNOX reduced intracellular glutamate and calcium responses and reduced invasion capacity in GBM. However, the invasion was not affected in GBM by

SUBMITTER: Lee M 

PROVIDER: S-EPMC11375122 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature