Determination of Mitochondrial Respiration and Glycolysis in Ex Vivo Retinal Tissue Samples.

ABSTRACT: Mitochondrial respiration is a critical energy-generating pathway in all cells, especially retinal photoreceptors that possess a highly active metabolism. In addition, photoreceptors also exhibit high aerobic glycolysis like cancer cells. Precise measurements of these metabolic activities can provide valuable insights into cellular homeostasis under physiological conditions and in disease states. High throughput microplate-based assays have been developed to measure mitochondrial respiration and various metabolic activities in live cells. However, a vast majority of these are developed for cultured cells and have not been optimized for intact tissue samples and for application ex vivo. Described here is a detailed step-by-step protocol, using microplate-based fluorescence technology, to di

SUBMITTER: Jiang K 

PROVIDER: S-EPMC11375468 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature