Allosteric Communication of the Dimerization and the Catalytic Domain in Photoreceptor Guanylate Cyclase.

ABSTRACT: Phototransduction in vertebrate photoreceptor cells is controlled by Ca²⁺-dependent feedback loops involving the membrane-bound guanylate cyclase GC-E that synthesizes the second messenger guanosine-3',5'-cyclic monophosphate. Intracellular Ca²⁺-sensor proteins named guanylate cyclase-activating proteins (GCAPs) regulate the activity of GC-E by switching from a Ca²⁺-bound inhibiting state to a Ca²⁺-free/Mg²⁺-bound activating state. The gene GUCY2D encodes for human GC-E, and mutations in GUCY2D are often associated with an imbalance of Ca²⁺ and cGMP homeostasis causing retinal disorders. Here, we investigate the Ca²⁺-dependent inhibition of the constitutively active GC-E mutant V902L. The inhibition

SUBMITTER: Shahu MK 

PROVIDER: S-EPMC11375764 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature