Selective Extraction Process and Characterization of Antioxidant Phenolic Compounds from Pereskia aculeata Leaves Using UPLC-ESI-Q-TOF-MS/MS.

ABSTRACT: This study innovates in comparing biological activities and chemical composition obtained from extracts and fractions from Pereskia aculeate leaves. Seven extracts and five fractions were produced by conventional successive solid-liquid extraction coupled with simultaneous bioguided purification using solvents of distinct polarities. A comparative analysis was conducted between these purified fractions and the original extracts to elucidate potential improvements in the bioactivity. The extract and fractions were evaluated using the ABTS, DPPH, FRAP, and Folin-Ciocalteau methods and HPLC-DAD and UHPLC-ESI-Q-TOF-MS/MS evaluated chemical composition. The fractions obtained from the hydroalcoholic extract showed better results, with the acetone fraction (Fr-Ace) exhibiting enhanced bio

SUBMITTER: Jacobsen SS 

PROVIDER: S-EPMC11375806 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature