Singlet oxygen detection in vivo is hindered by nonspecific SOSG staining.

ABSTRACT: Singlet oxygen is considered an important cell damaging agent due to its propensity to react with organic compounds. This drives the interest in developing methods for determination of ¹O₂. Simplicity of application and high sensitivity makes fluorescent probes a popular choice for in vivo ¹O₂ detection. Despite its proclaimed cell-impermeability, the commercially available Singlet Oxygen Sensor Green (SOSG) is widely applied to support assertions of ¹O₂ involvement in cell and tissue damage. Our investigation, however, demonstrate that different microbial species and cancer cells become fluorescent when exposed to SOSG under conditions which exclude generation of ¹O₂. Cells, permeabilized with chlorhex

SUBMITTER: Kadhem Z 

PROVIDER: S-EPMC11377423 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature