The histone H3K9 methyltransferase G9a regulates tendon formation during development.

ABSTRACT: G9a is a histone methyltransferase that catalyzes the methylation of histone 3 lysine 9 (H3K9), which is involved in the regulation of gene expression. We had previously reported that G9a is expressed in developing tendons in vivo and in vitro and that G9a-deficient tenocytes show impaired proliferation and differentiation in vitro. In this study, we investigated the functions of G9a in tendon development in vivo by using G9a conditional knockout (G9a cKO) mice. We crossed Sox9^Cre/+ mice with G9a^fl/fl mice to generate G9a^fl/fl; Sox9^Cre/+ mice. The G9a cKO mice showed hypoplastic tendon formation at 3 weeks of age. Bromodeoxyuridine labeling on embryonic day 16.5 (E16.5) revealed decreased cell proliferation in the tenocytes of G9a cKO mice. Immun

SUBMITTER: Wada S 

PROVIDER: S-EPMC11377446 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature