Spectral-based detection of chromatin loops in multiplexed super-resolution FISH data.

ABSTRACT: Involved in mitotic condensation, interaction of transcriptional regulatory elements and isolation of structural domains, loop formation has become a paradigm in the deciphering of chromatin architecture and its functional role. Despite the emergence of increasingly powerful genome visualization techniques, the high variability in cell populations and the randomness of conformations still make loop detection a challenge. We introduce an approach for determining the presence and frequency of loops in a collection of experimental conformations obtained by multiplexed super-resolution imaging. Based on a spectral approach, in conjunction with neural networks, this method offers a powerful tool to detect loops in large experimental data sets, both at the population and single-cell levels. The

SUBMITTER: Liefsoens M 

PROVIDER: S-EPMC11377450 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature