Lens-free on-chip 3D microscopy based on wavelength-scanning Fourier ptychographic diffraction tomography.

ABSTRACT: Lens-free on-chip microscopy is a powerful and promising high-throughput computational microscopy technique due to its unique advantage of creating high-resolution images across the full field-of-view (FOV) of the imaging sensor. Nevertheless, most current lens-free microscopy methods have been designed for imaging only two-dimensional thin samples. Lens-free on-chip tomography (LFOCT) with a uniform resolution across the entire FOV and at a subpixel level remains a critical challenge. In this paper, we demonstrated a new LFOCT technique and associated imaging platform based on wavelength scanning Fourier ptychographic diffraction tomography (wsFPDT). Instead of using angularly-variable illuminations, in wsFPDT, the sample is illuminated by on-axis wavelength-variable illuminations, rangin

SUBMITTER: Wu X 

PROVIDER: S-EPMC11377727 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature