Improved green and red GRAB sensors for monitoring spatiotemporal serotonin release in vivo.

ABSTRACT: The serotonergic system plays important roles in both physiological and pathological processes, and is a therapeutic target for many psychiatric disorders. Although several genetically encoded GFP-based serotonin (5-HT) sensors were recently developed, their sensitivities and spectral profiles are relatively limited. To overcome these limitations, we optimized green fluorescent G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB_5-HT) sensors and developed a red fluorescent GRAB_5-HT sensor. These sensors exhibit excellent cell surface trafficking and high specificity, sensitivity and spatiotemporal resolution, making them suitable for monitoring 5-HT dynamics in vivo. Besides recording subcortical 5-HT release in freely moving mice, we observed both uniform a

SUBMITTER: Deng F 

PROVIDER: S-EPMC11377854 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature