Overcoming Challenges in Oligonucleotide Therapeutics Analysis: A Novel Nonion Pair Approach.

ABSTRACT: Oligonucleotide therapeutics (OT) have emerged as promising drug modality for various intractable diseases. Recently, liquid chromatography-mass spectrometry (LC-MS) has been commonly employed for characterizing and quantifying OT in biological samples. Traditionally, the ion pairing-reverse phase (IP-RP) LC-MS method has been utilized in OT bioanalyses; however, this approach is associated with several limitations, including the memory effect and ion suppression effect of IP reagents. Therefore, this study aimed to develop a new RP-LC-MS method that eliminates the need for IP reagents. Our investigation revealed that ammonium bicarbonate was essential for the successful implementation of this nonIP-RP-LC-MS-based bioanalysis of OT. Moreover, the developed method demonstrated high versatil

SUBMITTER: Hayashi Y 

PROVIDER: S-EPMC11378278 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature