Obesity affects brain cortex gene expression in an APOE genotype and sex dependent manner.

ABSTRACT:

Objective

Obesity is the top modifiable risk factor for Alzheimer's disease. We hypothesized that high fat diet (HFD)-induced obesity alters brain transcriptomics in APOE-genotype and sex dependent manners. Here, we investigated interactions between HFD, APOE, and sex, using a knock-in mouse model of the human APOE3 and APOE4 alleles.

Methods

Six-month-old APOE3-TR and APOE4-TR mice were treated with either HFD or control chow. After 4 months, total RNA was extracted from the cerebral cortices and analyzed by poly-A enriched RNA sequencing on the Illumina platform.

Results

Female mice demonstrated profound HFD-induced transcriptomic changes while there was little to no effect in males. In females, APOE3 brains demonstrated about five times more HFD-induced transcripto

SUBMITTER: Pandit H 

PROVIDER: S-EPMC11379128 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature