Multiplex imaging of localized prostate tumors reveals altered spatial organization of AR-positive cells in the microenvironment.

ABSTRACT: Mapping the spatial interactions of cancer, immune, and stromal cell states presents novel opportunities for patient stratification and for advancing immunotherapy. While single-cell studies revealed significant molecular heterogeneity in prostate cancer cells, the impact of spatial stromal cell heterogeneity remains poorly understood. Here, we used cyclic immunofluorescent imaging on whole-tissue sections to uncover novel spatial associations between cancer and stromal cells in low- and high-grade prostate tumors and tumor-adjacent normal tissues. Our results provide a spatial map of single cells and recurrent cellular neighborhoods in the prostate tumor microenvironment of treatment-naive patients. We report unique populations of mast cells that show distinct spatial associations with M2

SUBMITTER: Ak C 

PROVIDER: S-EPMC11379676 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature