P65 signaling dynamics drive the developmental progression of hematopoietic stem and progenitor cells through cell cycle regulation.

ABSTRACT: Most gene functions have been discovered through phenotypic observations under loss of function experiments that lack temporal control. However, cell signaling relies on limited transcriptional effectors, having to be re-used temporally and spatially within the organism. Despite that, the dynamic nature of signaling pathways have been overlooked due to the difficulty on their assessment, resulting in important bottlenecks. Here, we have utilized the rapid and synchronized developmental transitions occurring within the zebrafish embryo, in conjunction with custom NF-kB reporter embryos driving destabilized fluorophores that report signaling dynamics in real time. We reveal that NF-kB signaling works as a clock that controls the developmental progression of hematopoietic stem and progenitor

SUBMITTER: Campbell CA 

PROVIDER: S-EPMC11379711 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature